Field and Greenhouse Analysis of Variation for Disease Resistance in Tobacco Somaclones

Abstract

Daub, M. E., and Jenns, A. E. 1989. Field and greenhouse analysis of variation for disease resistance in tobacco somaclones. Phytopathology 79:600-605. A total of 854 somaclones of two flue-cured tobacco cultivars were parent cultivar in black shank resistance, but many had greater resistance generated from protoplast cultures, and their progeny analyzed in to bacterial wilt than did NC2326. Most cultivar Coker 319 somaclones greenhouse and field tests for yield, leaf chemistry, and resistance to black were more susceptible than the parent cultivar to black shank but had shank, bacterial wilt, tobacco mosaic virus, and root knot (Meloidogyne bacterial wilt resistance similar to that of Coker 319. Variation in black incognita). Under the culture conditions established in this study, shank and bacterial wilt resistance was slight, and few somaclones had approximately 55% of the somaclones were not self-fertile. Progeny of the responses equivalent to those of the susceptible and resistant control somaclones had normal phenotype and did not differ significantly from the cultivars. However, conditions used in this study reduced the amount of parent cultivars in yield and leaf chemistry. Significant variation was found potential variation. No somaclones were identified with resistance to in resistance to black shank and bacterial wilt, two diseases for which the tobacco mosaic virus or M. incognita. We conclude that genetic variation parent cultivars have low levels of resistance. The variation that was occurred in the somaclones, that the magnitude of variation was slight, and observed in response to these diseases depended on the disease as well as the that the variation depended on both the genotype of the parent cultivar and genotype of the parent. Somaclones of cultivar NC2326 were similar to the the trait. Additional keywords: Nicotiana tabacum, Phytophthora parasitica var. nicotianae, Pseudomonas solanacearum, tissue culture.