Abstract
The seasonal relationships of vitamin B 12 , phytoplankton and nutrients were investigated by field and bioassay experiments in the Saronicos Gulf, Aegean Sea (Eastern Mediterranean Sea). The field data demonstrated a statistically significant negative relationship of vitamin B 12 with Chl α, diatoms, flagellates, phosphate and total inorganic nitrogen. The bioassay experiments were performed using Pavlova lutheri as a test organism and examined the combined effect of nutrients and vitamin B 12 on growth. The results supported the hypothesis that in the area of study vitamin B 12 might not be a limiting factor for phytoplanktonic algae but it certainly stimulates growth through a multiple nutritional mechanism
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