Fibronectin Regulates Latent Transforming Growth Factor-β (TGFβ) by Controlling Matrix Assembly of Latent TGFβ-binding Protein-1

Sarah L Dallas,Pitchumani Sivakumar,Carolyn J.P Jones,Qian Chen,Donna M Peters,Deane F Mosher,Martin J Humphries,Cay M Kielty

doi:10.1074/jbc.m410762200

Abstract

Fibronectin Regulates Latent Transforming Growth Factor-β (TGFβ) by Controlling Matrix Assembly of Latent TGFβ-binding Protein-1

Highlights

Recent evidence suggests that the binding of growth factors to the extracellular matrix (ECM)1 is a major mechanism for
By 2 days in culture (Fig. 1, d2), the fibronectin became organized into a fibrillar network in the ECM, and a small amount of LTBP1 was observed that co-localized with fibronectin
The co-localization of LTBP1 and fibronectin started to diverge until day 21, when LTBP1 became localized in fibrils that were clearly distinct from fibronectin

Summary

Introduction

Recent evidence suggests that the binding of growth factors to the extracellular matrix (ECM) is a major mechanism for. The percentage of free LTBP1 varies from 10 to 90%, depending on the cell type and the differentiation stage examined [6, 9, 16]. These observations raise the possibility that LTBP1 may have important functions that are independent of TGF␤ and may be related to its properties as an ECM protein. Multiple approaches were used to disrupt fibronectin assembly, and the effect on LTBP1 and TGF␤ incorporation was determined These studies highlight a novel role for fibronectin in the regulation of TGF␤ via LTBP1 interactions

Methods

Results

Conclusion