Abstract
The adsorption of fibronectin on gold, Ti-, and Ta-oxide surfaces is investigated by means of the quartz crystal microbalance with dissipation (QCM-D) technique. The surface chemistry (gold, Ti-, and Ta-oxide) is found to influence the frequency shift observed during adsorption of the fibronectin layer with the magnitude being Δ f Au > Δ f Ti-oxide ∼ Δ f Ta-oxide . Corresponding variations in the dissipation change normalised to frequency change ( Δ D / Δ f ) for the layer are observed. The QCM-D data are further analyzed by the random sequential adsorption (RSA) model, and adsorption rate parameter k a and footprint (a) determined, which supported the trend seen in the Δ f and Δ D / Δ f values. The value of k a found by the RSA modelling of the QCM-D resonance frequency data is found to match the ratio between the mass measured by QCM-D and the mass reported by optical techniques in literature. We conclude that comparison of the adsorption rate parameter ( k a ) obtained by RSA modelling of the QCM-D data with k a values obtained from RSA modelling of data obtained using optical techniques can be a route to determine the degree of hydration of the adsorbed protein layer.
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