Abstract
Methods OVCAR3 and A2780 are the two common cell lines that are used for ovarian cancer studies. The different invasion and migration abilities were observed by scratch tests and transwell experiments in our preliminary study. Gene chip was used to screen the expression gene in these two different cell lines, and then, the differentially expressed genes (at least 2-fold difference, P value < 0.05) were analyzed using KEGG. Result Fibronectin 1 (FN1) was found to be the most strongly correlated with the invasion and migration abilities of the OVCAR3 cells. Real-time PCR and FN1 knockout cell line was conducted and confirmed this finding. Based on the Oncomine database analysis, comparing with normal people, ovarian cancer patients exhibited high levels of FN1 expression. Additionally, higher FN1 expression was found in patients with higher FIGO stages of cancer. Conclusion FN1 could be a new biomarker for ovarian cancer detection and progress indicator.
Highlights
Ovarian cancer is the fifth leading cause of cancer-related deaths among women worldwide; early detection tests are relatively lacking
The results revealed that the OVCAR3 cells did express Fibronectin 1 (FN1) at a higher level than the A2780 cells (Figure 3(c)), which proved that FN1 plays an important role in cell migration and that a high level of FN1 expression indicates high tumor cell mobility
We found that these two different cell lines exhibit strongly different cell migration and invasion abilities
Summary
Ovarian cancer is the fifth leading cause of cancer-related deaths among women worldwide; early detection tests are relatively lacking. Most women with ovarian cancer are diagnosed in the late stages of the disease, which portends a poor prognosis [1, 2]. Current surveillance strategies, which are achieved by transvaginal ultrasound or serum tumor marker cancer antigen 125 (CA125) tests, are ineffective in detecting ovarian cancer at an early stage [3, 4]. The early detection and diagnosis of ovarian cancer have a very important role in the survival and prognoses of patients with epithelial ovarian cancer. In a variety of tumors, such as nasopharyngeal carcinoma, osteosarcoma, esophageal cancer, and ovarian cancer, FN1 is an important tumor-related gene [8,9,10,11]
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