Abstract
The objective of this study was to evaluate the effectiveness of various storage media at 20 °C in maintaining the viability of human periodontal ligament fibroblasts (HPLF) over time. HPLF were maintained at 20 °C in skim milk (SM), whole milk (WM), freshly prepared Hank's balanced salt solution (HBSS), Save-A-Tooth(r), natural coconut water (NCW), coconut water industrialized (ICW) and tap water (negative control) for 3, 6, 24, 48, 72, 96 and 120 h. Cells maintained in Minimal Essential Medium (MEM-37) at 37 °C served as a positive control. Cell viability was determined by MTT assay. Statistical analysis was performed by Kruskal-Wallis test and Scheffe test (α = 5%). From 24 h, NCW was significantly better in maintaining cell viability than all other tested storage media (p<0.05). SM and WM were significantly better than HBSS for up to 72 h. Save-A-Tooth(r) and ICW were the worst conservation storage media. In conclusion, the effectiveness of the tested storage media to maintain the viability of the periodontal ligament cells was as follows, in a descending order: NCW > MEM-37> SM and IM> HBSS> ICW > Save-A-Tooth(r)> tap water.
Highlights
Tooth avulsion is characterized by complete displacement of a tooth from its alveolar socket
At 6h, the efficacy of natural coconut water (NCW) was similar to whole milk (WM) and skim milk (SM) and superior to Hank’s balanced salt solution (HBSS), Save-A-Tooth’s HBSS (Save) and industrialized coconut water (ICW)
The first tested hypothesis was partially rejected because from 24 h onwards, NCW was significantly better than all other media for maintaining cell viability
Summary
Tooth avulsion is characterized by complete displacement of a tooth from its alveolar socket. Periodontal cells are subject to contamination and dehydration and may become necrotic (1). The success of tooth replantation could be improved if the tooth is replanted immediately (1,2). As immediate repositioning is not always possible, it is extremely important to choose a suitable storage medium for maintenance of the periodontal ligament fibroblasts (PDLF) viability (1,2). Hank’s balanced salt solution (HBSS), available in the Save-A-Tooth® system (Phoenix-Lazerus, Shartlesville, PA, USA), has been recommended as the storage medium to maintain PDLF viability (1). Some experiments have shown that it is a suitable medium for the storage of avulsed teeth for periods ranging from 3 to 72 h (2-7). A disadvantage of this solution is that it may not be readily available in locations where tooth avulsion is likely to occur
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