Fibroblast Growth Factor Receptor, a Novel Receptor for Vegetative Insecticidal Protein Vip3Aa.

Kun Jiang,Xiaoyue Hou,Zhanglei Cao,Tongtong Tan,Jun Cai,Lu Han

doi:10.3390/toxins10120546

Kun Jiang, Xiaoyue Hou + Show 4 more

Open Access

https://doi.org/10.3390/toxins10120546

Copy DOI

Journal: Toxins	Publication Date: Dec 18, 2018
Citations: 37	License type: CC BY 4.0

Affiliation: Nankai University, Shandong University

Abstract

Vegetative insecticidal proteins (Vips), which are secreted by some Bacillus thuringiensis strains during vegetative growth, exhibit high virulence to many pests. Vip3A proteins have been used commercially both in some bio-insecticides and in transgenic crops; however, compared with insecticidal crystal proteins, the mechanism of action of Vip3A is still unclear. In this work, we indicated that the fibroblast growth factor receptor-like protein (Sf-FGFR) from the membrane of Sf9 cells could bind to Vip3Aa. The interaction between Vip3Aa and Sf-FGFR was confirmed by pull-down assays and dot blotting experiment in vitro. The binding affinity between Vip3Aa and extracellular regions of Sf-FGFR (GST-FGFR-N) was determined by microscale thermophoresis assay (MST). Moreover, Vip3Aa-Flag could be co-immunoprecipitated with Sf-FGFR-V5 ex vivo. Furthermore, knockdown of Sf-FGFR gene in Sf9 cells resulted in reducing the mortality of those cells to Vip3Aa. In summary, our data indicated that Sf-FGFR is a novel receptor for Vip3Aa.

Highlights

Bacillus thuringiensis (Bt) is a gram-positive, soil-dwelling bacterium that naturally produces insecticidal crystal proteins (ICPs) during sporulation
Results confirmed that Sf-fibroblast growth factor receptors (FGFRs) from Sf9 cells is a novel receptor for Vip3Aa
The Vip3Aa protein used a bait; wemembrane-proteins identified the fibroblast growth could bind to Vip3Aa

Summary

Introduction

Bacillus thuringiensis (Bt) is a gram-positive, soil-dwelling bacterium that naturally produces insecticidal crystal proteins (ICPs) during sporulation. The ICPs are highly specific against a variety of insects, as well as nematodes, mites, protozoa, and some human-cancer cells [1,2]. They have been used worldwide by traditional spray approaches or transgenic crops [3,4]. After ingestion by a susceptible larva, the environment of the midgut promotes crystal solubilization, protoxin release, and toxin production. Toxins bind to the specific receptors on the brush border membrane of midgut cell that facilitate the formation of an oligomeric structure, followed by pores formed in the cell membrane, resulting in the death of the insect [2]. With the extensive applications of ICPs, the cases of insect resistance to them are constantly emerging [2,3,4]

Methods

Results

Conclusion