Abstract
Fibroblast growth factors (FGFs) transmit their signals through four transmembrane receptors that are designated FGFR1-4. Alternative splicing in the extracellular region of FGFR1-3 generates receptor variants with different ligand binding affinities. Thus two types of transmembrane receptors (IIIb and IIIc isoforms) have been identified for FGFR2 and FGFR3, and the existence of analogous variants has been postulated for FGFR1 based on its genomic structure. However, only a single full-length transmembrane FGFR1 variant (FGFR1-IIIc) has been identified so far. Here we describe the cloning of a full-length cDNA encoding FGFR1-IIIb from a mouse skin wound cDNA library. This receptor isoform was expressed at the highest levels in a subset of sebaceous glands of the skin and in neurons of the hippocampus and the cerebellum. FGFR1-IIIb was expressed in L6 rat skeletal muscle myoblasts and used in cross-linking and receptor binding studies. FGF-1 was found to bind the receptor with high affinity, whereas FGF-2, -10, and -7 bound with significantly lower affinities. Despite their apparently similar but low affinities, FGF-10 but not FGF-7 induced the activation of p44/42 mitogen-activated protein kinase in FGFR1-IIIb-expressing L6 myoblasts and stimulated mitogenesis in these cells, demonstrating that this new receptor variant is a functional transmembrane receptor for FGF-10.
Highlights
Fibroblast growth factors (FGFs)1 comprise a growing family of structurally conserved mitogens, which currently includes 19 different members [1,2,3,4,5,6,7,8,9,10]
By analogy to FGFR2, IIIb and IIIc splice variants have been identified for FGFR3 [17], whereas this type of alternative splicing does not occur with the FGFR4 transcripts [22]
Differential Expression of FGFR1-IIIb, FGFR1-IIIc, and FGFR2-IIIb in Adult Mouse Tissues—In an attempt to isolate the full-length cDNA of murine FGFR1-IIIb, we first determined the optimal source for the corresponding mRNA
Summary
Fibroblast growth factors (FGFs)1 comprise a growing family of structurally conserved mitogens, which currently includes 19 different members [1,2,3,4,5,6,7,8,9,10]. Despite their apparently similar but low affinities, FGF-10 but not FGF-7 induced the activation of p44/42 mitogen-activated protein kinase in FGFR1-IIIb-expressing L6 myoblasts and stimulated mitogenesis in these cells, demonstrating that this new receptor variant is a functional transmembrane receptor for FGF-10.
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