Abstract
Background: Stem cells from human exfoliated deciduous teeth (SHEDs) are considered one of the most convenient sources of adult stem cells. This study aimed to examine the effect of fibroblast growth factor 6 (FGF-6) on SHEDs and evaluate CDK2 and MATK gene expression in SHED-derived microvesicles (MVs). SHEDs were cultured from deciduous teeth pulp. Methods: SHEDs were divided into two groups: the control group and test groups, with and without FGF-6 supplementation, respectively. After the third passage, SHED proliferation was assessed by MTT assay. MVs were purified and CDK2 and MATK gene expression was assessed by real-time polymerase chain reaction. SHEDs were identified by their positivity for CD90 and CD73, and negativity for CD45 and CD34. Results: SHEDs proliferation in the test group was significantly higher than in the control group (P<0.001). mRNA from SHED-derived MVs from the test group exhibited a markedly elevated expression of CDK2 and MATK, (P<0.002 and P<0.005, respectively) in comparison with those of the control group. FGF-6 enhanced the proliferation of SHEDs. Proliferation enhancement is favorable for the production of a large number of stem cells, which will then be beneficial for cell-based therapies. Conclusions: CDK2 and MATK genes in SHED-derived MVs can be used as molecular biomarkers for SHED proliferation.
Highlights
Stem cells from human exfoliated deciduous teeth (SHEDs) are considered one of the most convenient sources of adult stem cells
stem cells isolated from human deciduous teeth (SHEDs) were identified as ectomesenchymal stem cells through the quantification of several expressed mesenchymal stem cell markers using flow cytometry; they were shown to be positive for CD90 and CD73, and negative for CD45 and CD3419
The present study showed an increased expression of Cyclin-dependent kinase 2 (CDK2) and megakaryocyte-associated tyrosine kinase (MATK) genes present in RNA of microvesicles derived from SHEDs after Fibroblast growth factors (FGFs) -6 supplementation
Summary
Stem cells from human exfoliated deciduous teeth (SHEDs) are considered one of the most convenient sources of adult stem cells. This study aimed to examine the effect of fibroblast growth factor 6 (FGF-6) on SHEDs and evaluate CDK2 and MATK gene expression in SHED-derived microvesicles (MVs). Methods: SHEDs were divided into two groups: the control group and test groups, with and without FGF-6 supplementation, respectively. SHED proliferation was assessed by MTT assay. MVs were purified and CDK2 and MATK gene expression was assessed by real-time polymerase chain reaction. Results: SHEDs proliferation in the test group was significantly higher than in the control group (P
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