Abstract
We investigated fibroblast growth factor 12 (FGF12) as a transcript enriched in the inner ear by searching published cDNA library databases. FGF12 is a fibroblast growth factor homologous factor, a subset of the FGF superfamily. To date, its localisation and function in the inner ear have not been determined. Here, we show that FGF12 mRNA is localised in spiral ganglion neurons (SGNs) and the vestibular ganglion. We also show that FGF12 protein is localised in SGNs, the vestibular ganglion, and nerve fibres extending beneath hair cells. Moreover, we investigated FGF12 function in auditory and vestibular systems using Fgf12-knockout (FGF12-KO) mice generated with CRISPR/Cas9 technology. Our results show that the inner ear morphology of FGF12-KO mice is not significantly different compared with wild-type mice. However, FGF12-KO mice exhibited an increased hearing threshold, as measured by the auditory brainstem response, as well as deficits in rotarod and balance beam performance tests. These results suggest that FGF12 is necessary for normal auditory and equilibrium function.
Highlights
We investigated fibroblast growth factor 12 (FGF12) as a transcript enriched in the inner ear by searching published cDNA library databases
We found that FGF12 mRNA was abundantly expressed in the cochlea and vestibular ganglion (Fig. 1a). β-Actin was used as an internal control
We found that FGF12 mRNA is abundantly expressed in the inner ear (Fig. 1) and localised in the vestibular and spiral ganglia (Fig. 2)
Summary
We investigated fibroblast growth factor 12 (FGF12) as a transcript enriched in the inner ear by searching published cDNA library databases. FGF12-KO mice exhibited an increased hearing threshold, as measured by the auditory brainstem response, as well as deficits in rotarod and balance beam performance tests These results suggest that FGF12 is necessary for normal auditory and equilibrium function. Recent studies of transcripts enriched in the inner ear have provided novel insight into hereditary hearing loss and normal auditory function[3,4]. We focused on fibroblast growth factor 12 (FGF12) as an inner ear-enriched transcript and performed experiments to investigate its localisation and function. Auditory and vestibular function was examined in the FGF12-KO mice that we generated This is the first report to show FGF12 function in the inner ear, and our findings provide novel insight into the contribution of FHF to normal auditory and vestibular function
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