Fibrinogen Adsorption on the Lipid Surface as a Factor of Regulation of Fibrin Formation

Abstract

It was found that the enzymes of the coagulation cascade interact with lipid microparticles or microvesicles of natural or artificial origin that circulate in the blood. Such interactions can modulate hemostasis. Less is known about the consequences of direct interaction of fibrinogen/fibrin with microvesicles. It was shown in this study with purified systems that the presence of lipid particles of various compositions in a solution can specifically affect the kinetics of fibrin polymerization. Experimental data are presented that indicate that these effects are due to the adsorption of fibrinogen on the surface of microvesicles. In particular, it was shown that the adsorption of fibrinogen at a sufficiently high concentration led to an acceleration of thrombin-induced fibrin polymerization due to the local concentrating effect and the inclusion of microvesicles in the clot structure. Thus, the direct interaction of fibrinogen with microvesicles is a significant factor of hemostasis, which should be considered in the analysis of blood clotting disorders and the development of artificial lipid carriers of drugs.