Abstract
Fibrin glue has been used clinically for decades in a wide variety of surgical specialties and is now being investigated as a medium for local, prolonged drug delivery. Effective local delivery of antibacterial substances is important perioperatively in patients with implanted medical devices or postoperatively for deep wounds. However, prolonged local application of antibiotics is often not possible or simply inadequate. Biofilm formation and antibiotic resistance are also major obstacles to antibacterial therapy. In this paper we test the biocompatibility of bacteriophages incorporated within fibrin glue, track the release of bacteriophages from fibrin scaffolds, and measure the antibacterial activity of released bacteriophages. Fibrin glue polymerized in the presence of the PA5 bacteriophage released high titers of bacteriophages during 11 days of incubation in liquid medium. Released PA5 bacteriophages were effective in killing Pseudomonas aeruginosa PA01. Overall, our results show that fibrin glue can be used for sustained delivery of bacteriophages and this strategy holds promise for many antibacterial applications.
Highlights
Effective local delivery of antibacterial substances is important perioperatively in patients with implanted medical devices or postoperatively for deep wounds
Bacteriophages embedded in fibrin glue could serve to treat or prevent infections in many surgical fields, especially those infections associated with antibiotic resistant bacteria or biofilms
We found that bacteriophages were well distributed within fibrin glue scaffolds when embedded into the fibrin glue
Summary
Effective local delivery of antibacterial substances is important perioperatively in patients with implanted medical devices or postoperatively for deep wounds. A number of materials such as polymethylmethacrylate, collagen, chitosan, and polyethylene glycol have been loaded with antibiotics and applied intraoperatively thereby conferring a prolonged local antimicrobial effect[1,2,3,4] Such a local drug delivery approach is especially important for patients with implanted cardiovascular devices because of the high mortality and healthcare costs associated with infectious complications[5,6]. Fibrin glue is a two-component hemostat, sealant, and tissue adhesive consisting of fibrinogen and thrombin It has been used clinically for decades in a wide variety of surgical specialties and has several newer uses in cell and drug delivery[13]. Bacteriophages embedded in fibrin glue could serve to treat or prevent infections in many surgical fields, especially those infections associated with antibiotic resistant bacteria or biofilms
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
