Abstract
An enzyme optrode capable of continuously monitoring glucose concentrations is presented. Glucose oxidase was physically immobilised in a sensing layer at the end of a fibre optic light guide. The enzyme catalyses the oxidation of glucose to give gluconic acid, which, in turn, lowers the pH in the microenvironment of the sensor. The enzymatic reaction can therefore be monitored by following the changes in the fluorescence of a pH-sensitive dye incorporated into the sensing layer. Flow-through measurements were performed in buffer solutions of various strength. The response time for 90% of the total signal change was 8–12 min, and 0.1–2mM glucose was found to be detectable. Saturation was reached at glucose concentrations of 2–3 mM.
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