Abstract
Xanthine is produced by guanine deaminase and xanthine oxidase from guanine and hypoxanthine, respectively. It serves as an indicator of spoilage of meat and various pathological conditions. An optical biosensor has been fabricated for the detection of xanthine by absorption transmission approach. The bioassay principle used in this study is conversion of xanthine to uric acid and H2O2 by XO. Due to the production of uric acid, pH of the reaction decreases and falls in range of 7.5 - 6. The absorptive dye phenol red has been screened to visualize the change in colour. The coimmobilization of XO and dye phenol red indicator was done with sol-gel method onto the circular plastic discs for the development of biosensor. Volume of sample required for detection has been miniaturized to 10μl. The linear range of concentration and limit of detection were 0.5μM – 150μM and 0.5 μM respectively, have been achieved for xanthine. The designed biosensor was successfully employed for the detection of xanthine in chicken meat sample. Present study leads to the development of a fast and dependable biosensor with miniaturized sample volume and also mass scale screening possible through colour visualization.
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