Abstract
Fibre-optic biosensors were constructed for determination of hypoxanthine and xanthine. Xanthine oxidase and peroxidase were immobilized on different preactivated membranes which were subsequently mounted onto the tip of a fibre-optic bundle. The H 2O 2 generated by the reaction of hypoxanthine and xanthine oxidase was measured by chemiluminescence (CL) detection using luminol and peroxidase. A linear calibration curve of the sensors in the range of 1–316 μM hypoxanthine and 3ṡ1–216 μM xanthine, respectively, with a detection limit of 0ṡ55 μM hypoxanthine was obtained. Recovery of hypoxanthine ranged between 91 and 102%.
Published Version
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