Abstract
High-quality cotton fibre equates to a more comfortable textile. Fibre length is an important index of fibre quality. Hydrogen peroxide (H2O2) acts as a signalling molecule in the regulation of fibre elongation. Results from in vitro ovule culture suggest that the alteration of fibre cell H2O2 levels affects fibre development. Ascorbate peroxidase (APX) is an important reactive oxygen species (ROS) scavenging enzyme, and we found that GhAPX1AT/DT encoded one member of the previously unrealized group of cytosolic APXs (cAPXs) that were preferentially expressed during the fibre elongation stage. Transgenic cottons with up- and down-regulation of GhAPX1AT/DT were generated to control fibre endogenous levels of H2O2 Suppression of all cAPX (IAO) resulted in a 3.5-fold increase in H2O2 level in fibres and oxidative stress, which significantly suppressed fibre elongation. The fibre length of transgenic lines with over-expression or specific down-regulation of GhAPX1AT/DT did not show any obvious change. However, the fibres in the over-expression lines exhibited higher tolerance to oxidative stress. Differentially expressed genes (DEGs) in fibres at 10 days post-anthesis (DPA) of IAO lines identified by RNA-seq were related to redox homeostasis, signalling pathways, stress responses and cell wall synthesis, and the DEGs that were up-regulated in IAO lines were also up-regulated in the 10 DPA and 20 DPA fibres of wild cotton compared with domesticated cotton. These results suggest that optimal H2O2 levels and redox state regulated by cytosolic APX are key mechanisms regulating fibre elongation, and dysregulation of the increase in H2O2 induces oxidative stress and results in shorter fibres by initiating secondary cell wall-related gene expression.
Highlights
The list of total reads mapped to G. raimondii Genome multi-position match oot Stem Leaf Petal Anther Stigma Ovule 0 DPA Fiber 10 DPA Fiber 20 DPA Ovule 10 DPA Ovule 20 DPA
T0 10 DPA Inserted Seed T1/2/3 NO
Summary
Used for RT-PCR/Northe rn 35s-GhAPX1/O RF-RNAi 35s-GhAPX1/O RF-RNAi 3'UTR-RNAi realtime PCR realtime PCR Northern realtime PCR realtime PCR realtime PCR realtime PCR realtime PCR realtime PCR realtime PCR realtime PCR realtime PCR realtime PCR realtime PCR Cupredoxin-S Cupredoxin-AS POD-S POD-AS SHSP1-S SHSP1-AS SHSP2-S SHSP2-AS SHSP3-S SHSP3-AS SHSP4-S SHSP4-AS SHSP5-S SHSP5-AS SHSP6S SHSP6AS HSP70-S HSP70-AS Cysteine proteinases-S Cysteine proteinases-AS DRL1-S DRL1-AS TP1-S TP1-AS LEA5-S LEA5-AS MTN3-S MTN3-AS OS34-S OS34-AS SAM-S SAM-AS ACO-S ACO-AS BZIP44-S BZIP44-AS PAR1-S PAR1-AS YABBY-S YABBY-AS FLA7-S FLA7-AS HPRG1-S HPRG1-AS
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