Abstract
A compact and flexible fiber-optic cell for absorbance detection in capillary electrophoresis (CE) has been developed. Optical fibers with a large core diameter were combined with focusing optics and a circular aperture, the size of the aperture being matched to the I.D. and O.D. of the separation column, in order to maximize light throughput and reduce stray light. The detection limit for mesityl oxide was 1.5 μ M for a 100-μm I.D. column, and the linear dynamic range was larger than 1000 for columns from 100-μm I.D. down to 30-μm I.D. The design is suitable for high-speed CE, utilizing short columns.
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