Abstract
Bundled fiber optics allow in vivo imaging at deep sites in a body. The intrinsic optical contrast detects detailed structures in blood vessels and organs. We developed a bundled-fiber-coupled endomicroscope, enabling stereoscopic three-dimensional (3-D) reflectance imaging with a multipositional illumination scheme. Two illumination sites were attached to obtain reflectance images with left and right illumination. Depth was estimated by the horizontal disparity between the two images under alternative illuminations and was calibrated by the targets with known depths. This depth reconstruction was applied to an animal model to obtain the 3-D structure of blood vessels of the cerebral cortex (Cereb cortex) and preputial gland (Pre gla). The 3-D endomicroscope could be instrumental to microlevel reflectance imaging, improving the precision in subjective depth perception, spatial orientation, and identification of anatomical structures.
Highlights
Bundled fiber optics allow in vivo imaging at deep sites in a body
The experimental setup consisted of an objective lens (×20, NA 0.5), a tube lens (f 1⁄4 180) optically coupled to the fiber bundle (DP-SMD002; TAC), and a cooled EM-CCD camera (−90 Æ 5°C, iXon; Andor Technology) [Fig. 1(c)]
Microbeads and an ink-mark printed on a transparent polyester (PET) film with a known thickness were used to confirm the ability of the multipositional illumination scheme to detect depth
Summary
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