FIBCD1 binds to Aspergillus fumigatus PAMPs and modulates the inflammatory response.

Abstract

Event Abstract Back to Event FIBCD1 binds to Aspergillus fumigatus PAMPs and modulates the inflammatory response. Christine S. Jepsen1*, Lalit K. Dubey1, Jesper B. Moeller1, Mark A. Hammond1, Ole Nielsen2, Anders Schlosser1, Grith L. Sorensen1 and Uffe Holmskov1 1 University of Southern Denmark, Department of Cardiovascular and Renal Research, Denmark 2 Odense University Hospital, Department of Pathology, Denmark We have recently identified and characterised fibrinogen C domain-containing protein 1 (FIBCD1) as a homotetrameric type II transmembrane protein expressed by epithelial cells. FIBCD1 is an endocytic receptor that binds crab chitin and other acetylated compounds calcium- and acetate-dependently through the C-terminal fibrinogen-related domain (FReD). In the present study, we investigate the role of FIBCD1 in the immune reaction against Aspergillus fumigatus (Afu), a pathogenic fungus involved in the development of asthma and allergy in western society. We show that FIBCD1 binds the Afu cell wall alkaline-insoluble fraction (AIF), which is primarily composed of chitin, beta-glucan, and galactomannan, in a calcium- and acetate-independent manner. We demonstrate that FIBCD1 recognises chitin-rich zones in the fungal cell wall in different cellular stages of Afu and that FIBCD1 is expressed in ciliated airway epithelial cells from patients with aspergillosis. We show that conidia, AIF, chitin, and beta-glucan induce IL-8 secretion from A549 lung epithelial cells in a time- and dose-dependent manner. Furthermore, our results indicate that FIBCD1-transfected A549 cells display reduced IL-8 secretion compared to sham-transfected A549 cells when stimulated with any of these compounds and that this reduction is significantly increased when the compound is a FIBCD1 ligand. Collectively, these results indicate that FIBCD1 is present in human airway epithelial cells, binds Afu cell wall PAMPs, and influences the inflammatory response in the lung. Keywords: Aspergillus fumigatus, FIBCD1, airway, IL-8, Chitin Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Jepsen CS, Dubey LK, Moeller JB, Hammond MA, Nielsen O, Schlosser A, Sorensen GL and Holmskov U (2013). FIBCD1 binds to Aspergillus fumigatus PAMPs and modulates the inflammatory response.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01139 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 31 Jul 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Christine S Jepsen, University of Southern Denmark, Department of Cardiovascular and Renal Research, Odense, Denmark, cesj222@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Christine S Jepsen Lalit K Dubey Jesper B Moeller Mark A Hammond Ole Nielsen Anders Schlosser Grith L Sorensen Uffe Holmskov Google Christine S Jepsen Lalit K Dubey Jesper B Moeller Mark A Hammond Ole Nielsen Anders Schlosser Grith L Sorensen Uffe Holmskov Google Scholar Christine S Jepsen Lalit K Dubey Jesper B Moeller Mark A Hammond Ole Nielsen Anders Schlosser Grith L Sorensen Uffe Holmskov PubMed Christine S Jepsen Lalit K Dubey Jesper B Moeller Mark A Hammond Ole Nielsen Anders Schlosser Grith L Sorensen Uffe Holmskov Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.