Abstract
We show here that Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5′-monophosphates from such natural metabolites as adenosine 5′-phosphosulfate and adenosine 5′-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 5′- O-phosphorofluoridate and adenosine 5′- O-(γ-fluorotriphosphate), and release AMP from them. With respect to the former, Fhits behave like a phosphodiesterase I concomitant with cleavage of the P–F bond. Some kinetic parameters and implications of the novel reactions catalyzed by the human and plant ( Arabidopsis thaliana) Fhit proteins are presented.
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