Abstract
Taste buds develop in different regions of the mammal oral cavity. Adult stem cells in various organs including the tongue papillae are marked by leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5) and its homolog, Lgr6. Recent studies have reported that adult taste stem/progenitor cells in circumvallate papilla (CVP) on the posterior tongue are Lgr5-positive. In this study, we confirm the Lgr5 expression pattern during CVP development. A previous study reported that mesenchymal Fgf10 is necessary for maintaining epithelial Lgr5-positive stem/progenitor cells. To confirm the interaction between Lgr5-positive CVP epithelium and mesenchymal factor FGF10, reverse recombination (180-degree) was performed after tongue epithelium detachment. FGF10 protein-soaked bead implantation was performed after reverse recombination to rescue CVP development. Moreover, we reduced mesenchymal Fgf10 by BIO and SU5402 treatment which disrupted CVP morphogenesis. This study suggests that the crosstalk between epithelial Lgr5 and mesenchymal Fgf10 plays a pivotal role in CVP epithelium invagination during mouse tongue CVP development by maintaining Lgr5-positive stem/progenitor cells.
Highlights
Taste buds develop in different regions of the oral cavity in mammals
Lingual papillae consist of four kinds of papillae: FFPs, filiform papillae, FOPs, and circumvallate papilla (CVP), which all localize to the surface of the tongue
All types of lingual papillae have a specific region on the tongue in which they are formed; the CVP is localized to the posterior region of the tongue (Norton and Netter, 2012)
Summary
Taste buds develop in different regions of the oral cavity in mammals. Taste buds localize in three different types of taste papillae on the mammalian tongue. The anterior dorsum of the tongue is covered with fungiform papillae (FFPs). Circumvallate papillae (CVPs) are localized medially on the posterior portion of the tongue. Foliate papillae (FOPs) are located laterally on the posterior side of the tongue (Kist et al, 2014). Different taste bud subtypes may have different longevities (Beidler and Smallman, 1965; Hamamichi et al, 2006; Perea-Martinez et al, 2013; Mistretta and Kumari, 2017)
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