Abstract
Immune serum was prepared in rabbits by injection of purified fetuin. Since the antiserum was found to be immunochemically homogeneous and was shown to be directed against fetuin, it was used to estimate quantitatively fetuin in fetal and adult bovine sera. The ratio of fetuin to total serum proteins was found to be 0.4–0.5 and did not vary significantly during development of the fetus. Fetuin was found in adult sera but at markedly lower levels. Cross reactions of fetuin antiserum with fetal and adult goat and sheep sera have been found. By various criteria, these heterologous antigens appeared to be quite similar, but not identical, to the protein derived from fetal calf serum. No cross reactions were observed with the sera of several other species tested. Fetuin from which the terminal sialic acid residues were removed reacted to a greater extent than the original antigen with fetuin antibody in the quantitative complement fixation assay. Glycopeptides derived from fetuin by proteolytic digestion did not react with fetuin antiserum, nor did they inhibit to any significant extent complement fixation of native fetuin with its homologous antibody.
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