Fetuin-A (α2HS-Glycoprotein) Is a Major Serum Adhesive Protein That Mediates Growth Signaling in Breast Tumor Cells

Amos M Sakwe,Rainelli Koumangoye,Shawn J Goodwin,Josiah Ochieng

doi:10.1074/jbc.m110.128926

Amos M Sakwe, Rainelli Koumangoye + Show 2 more

Open Access

https://doi.org/10.1074/jbc.m110.128926

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Abstract

Fetuin-A (α2HS-Glycoprotein) Is a Major Serum Adhesive Protein That Mediates Growth Signaling in Breast Tumor Cells

Highlights

Serum, fetal bovine serum, is widely used as a supplement in culture media that is required for growth of most cells in culture
To revisit the question of whether or not fetuin-A plays any role in cellular adhesion and signaling, we developed a purification protocol in which the less pure Pedersen preparation was further purified by glycerol gradient centrifugation
Ultrafiltration and Wheat Germ Agglutinin Purification of Pedersen Fetuin-A—The rationale behind using ultrafiltration as a strategy to further purify Pedersen fetuin-A was to separate uncomplexed fetuin-A from its high molecular weight aggregates formed with its contaminants

Summary

Introduction

Fetal bovine serum, is widely used as a supplement in culture media that is required for growth of most cells in culture (for a review, see Ref. 1). We resolved human serum on a glycerol gradient and determined that cell attachment and growth are associated with the fractions that contain ahsg/fetuin-A. Tumor cells that lacked annexin A6 (BT-A6-sh), one of the cell surface receptors for fetuin-A [15], had the poorest adhesion to the three pooled fractions and to Pedersen fetuin-A from two independent vendors (Sigma and Calbiochem) (Fig. 3D).

Results

Conclusion