Fetal bovine serum adsorption onto hydroxyapatite sensor monitoring by quartz crystal microbalance with dissipation technique

Abstract

Adsorption behavior of fetal bovine serum (FBS) onto hydroxyapatite (HAp) was analyzed in situ in phosphate buffer saline (PBS), minimum essential mediums (αMEM or DMEM), and different carbonate-concentrated buffers by using a quartz crystal microbalance with dissipation (QCM-D) technique. Although the adsorption behaviors of FBS onto gold showed no dependence on the solvents, those onto HAp clearly depended on the adsorbed ions in the solvents (PBS or MEMs). The amount and viscoelasticity of FBS adsorbed onto HAp in PBS were approximately two times larger than those in MEMs. The adsorption amounts of carbonate ions on HAp in the carbonate buffers increased with increasing carbonate concentrations, which inhibited the adsorption of FBS and decreased the viscoelastic property of the adsorption layers.