Fetal and Neonatal Diastolic Myocardial Strain Rate: Normal Reference Ranges and Reproducibility in a Prospective, Longitudinal Cohort of Pregnancies

Abstract

Normative fetal diastolic strain rate values have not been comprehensively reported. The aims ofthis study were to report normative data for diastolic strain rate parameters across gestation and upon delivery, determine the effect of advancing gestational age on these measures, and compare interobserver variability at multiple gestational age time points. Sixty gravid women were enrolled before 20weeks' gestation. The following measures were obtained by two blinded observers at five time points across gestation and at 4 to 8weeks' postnatal age: global left ventricular circumferential strain rate peak E and A waves, global left and right ventricular longitudinal strain rate peak E and A waves, and mitral and tricuspid valve E/A ratios. Reproducibility was assessed using intraclass correlation and Bland-Altman analysis. Least square means analysis was used to evaluate for changes across gestational age. Left ventricular longitudinal and circumferential diastolic strain rate values decreased across gestation, while right ventricular longitudinal values remained stable. With delivery, left ventricular values remained fairly stable, while right ventricular values increased. Intraclass correlations for diastolic strain rate values were 0.68 to 0.94 at ≥24weeks' gestation and 0.25 to 0.82 for values at 20 to 21weeks' gestation. Intraclass correlations ranged from 0.49 to 0.90 for mitral valve and tricuspid valveE/A ratios across gestation. Fetal measures of diastolic strain rate have acceptable reproducibility after 24weeks' gestation. The described changes across gestation likely reflect intrinsic myocardial adaptation to loading conditions. These reference ranges can be used to assess effects of various disease states on fetal myocardial deformation.