Festphasensynthese von Oligodesoxyribonucleotiden an Polystyren‐Teflon‐Trägern nach der Diestermethode

Abstract

Phosphodiester Solid Phase Synthesis of Oligodeoxyribonucleotides on Polystyrene‐Teflon‐SupportsPhosphodiester solid phase method on two different polystyrene‐teflon‐supports has been applied for for the rapid synthesis of the hexanucleotides d(TCTAGA), d(TGTCAA), d(TTGACA) and the octanucleotides d(CATTATAC) and d(GTATAATG)Als Abkürzungen für die Nucleinsäurebausteine und deren Schutzgruppen wurden die von der IUPAC‐IUB Commission on Biochemical Nomenclature (CBN) empfohlenen Regeln verwendet, vgl. Hoppe‐Seyler's Z. Physiol. Chem. 351, 1055 (1970); außerdem wurden abgekürzt:DEAE = O‐(Diethylamino)ethyl, HPLC = Hochdruckchromatographie, O.D.E. = optische Dichteeinheit [17], TEAB = Triethylammoniumbicarbonat, TFA = Trifluoressigsäure, Tris = α,α,α‐Tris‐(hydroxymethyl)‐methyl‐amin , respectively. In the case of hexanucleotides a polymeric support with 90 mg polystyrene/g support has been used. The octanucleotides were prepared using a 180 mg polystyrene/g support. The yields of all oligonucleotides were 42–51%/condensation step.