Abstract
Objective:To explore the promoting apoptosis and antitumor activities of ferulic acid (FA) in human osteosarcoma and its potential mechanism.Methods:The SaOS-2 and MG63 osteosarcoma cell lines were opted to experiment and these cells were, respectively, cultured with various concentrations of FA (0 μM, 10 μM, 20 μM, 40 μM) for 72 hours at 37°C. The viabilities of the FA treated cells were monitored by MTT. Apoptosis cells were evaluated using annexin V/PI by flow cytometry. Apoptosis proteins caspase-3, procaspase-3, Bcl-2 and Bax were detected by western blot. Expressions of apoptotic genes Bcl-2 and Bax were quantified by qPCR.Results:The cell viabilities were critically declined in the concentration-dependent manner in FA groups (P < 0.01). The apoptosis cells were increased proportionately with the concentration of FA (P < 0.05). The procaspase-3 protein contents, and Bcl-2 mRNA and protein contents were significantly decreased while caspase-3 protein contents, and Bax mRNA and protein contents were concomitantly increased in the concentration-dependent manner in FA groups (P < 0.05). The response to FA by the SaOS-2 osteosarcoma cell was similar with the MG63 osteosarcoma cell (P > 0.05).Conclusion:Ferulic acid could significantly descend osteosarcoma cell viability through the promoting apoptosis pathway in which FA activates both caspase-3 and Bax and inactivates Bcl-2.
Highlights
Osteosarcoma, an intra-osseous malignant neoplasm, has a bimodal age distribution of incidence which is comprised of an adolescent peak encountered in about age 15 and an elderly peak encountered in about age 75.1 The prognoses of1
In this study we investigate the influences of ferulic acid (FA) on apoptosis in osteosarcoma cell lines
The apoptotic cells detected by flow cytometry had demonstrated that there were significantly increased in the population of apoptotic cells of the FA group compared to the control group (P < 0.05)
Summary
Osteosarcoma, an intra-osseous malignant neoplasm, has a bimodal age distribution of incidence which is comprised of an adolescent peak encountered in about age 15 and an elderly peak encountered in about age 75.1 The prognoses of. FA (4-hydroxy-3-methoxycinnamic acid) is extracted from some natural plants, such as Asafoetida giantfennel, Angelica sinensis, Cimicifuga racemosa, Glycyrrhiza uralensis, and Ligusticum chuanxiong, which have been employed as the traditional Chinese herbs to treat multiple diseases for several thousand years.[3,4,5] Recently, numerous literatures have demonstrated the antitumor activities and protective effects of FA on thyroid cancer, prostate cancer, skin carcinogenesis, lung cancer, etc.[6,7,8,9] Whereas the antitumor activities and its mechanism of FA in osteosarcoma remain still under discussion. Resulting in an active and programmed demise of the cell, is originally induced by all sorts of physiological and pathological stimuli, including a broad variety of naturally occurring molecules, especially extractions from plants. In this study we investigate the influences of FA on apoptosis in osteosarcoma cell lines
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