Abstract
RATIONALE: Because phenolic compounds can precipitate or complex with proteins, we postulated that interactions of phenolics with IgE antibodies help enhance IgE binding to peanut allergens in Western blots. METHODS: Three different phenolics such as ferulic, caffeic and chlorogenic acids were examined. Each was mixed with a diluted pooled plasma from peanut-allergic patients, and incubated with a PVDF membrane containing major peanut allergens transferred from SDS-PAGE. The membrane was probed with a goat anti-human IgE peroxidase and o-phenylenediamine. A control without phenolics was also performed. RESULTS: Of the phree phenolics tested, only ferulic acid was able to enhance the density of allergen bands on the membrane and reduce the color development time. In this case, the ferulic acid protocol was approximately 6 times faster than the control. CONCLUSIONS: Feulic acid in complexation with IgE antibodies enhanced IgE binding to peanut allergens in Western blots. The improved ferulic protocol was approximately 6 times faster than the control.
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