Abstract
We report regeneration of fertile plants from barley (H. vulgale L. cv. Igri) protoplasts isolated from long-term cultures of embryogenic suspension cells. Calli induced from immature embryos were transferred into liquid medium, and suspension cult.ures were established. Protoplasts isolated from suspension cells divided and developed colonies. Somatic embryogenesis from colonies was observed on medium for plant regeneration. These embryogenic structures were transferred to fresh regeneration medium, and subsequently, green or albino shoots were regenerated in four cel] Iines. One of t.hese lines yielded highly regenerable protoplasts even after 9-3 months of subculture. The green shoots regenerated ¥vere transferred to hormone-free medium, and then, plantlets that developed strong root systems were potted in soii. After two monthsof vernalization, these plants were grown in a growth chamber and set. seeds. .
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