Abstract
We are optimizing a ferrozine‐based assay to analyze the iron content in Drosophila S2 cells. We hypothesize that putative ferric reductases CG1275 or CG8399 may be involved in reducing iron in the hemolymph and allowing uptake into cells. We have modified a ferrozine‐based assay, in which ferrozine complexes with ferrous ions in solution and forms a stable magenta complex species that can be detected at 562 nm. Using this method with an Ultraspec II spectrophotometer we obtained a limit of detection of 0.30 μg/mL. This method provides a reliable, inexpensive and simple way of analyzing and quantifying the iron content within the S2 cells, allowing us to analyze iron content after RNA interference experiments to knock down CG1275 and CG8399.Support or Funding InformationNSF IOS‐1656407This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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