Abstract
We have assessed iron acquisition by Laccaria laccata ATCC 42217 growing in defined medium with adequate levels of iron to support growth. A series of enzyme tests were conducted with mycelium to establish optimum conditions for ferric citrate reductase activity and to assess enzyme ihibition by divalent cations and by redox‐active metal oxyanions. Greatest reduction of ferric ion by these fungal cells was observed at pH 6.0 when NADH and FMN were added. Riboflavin was found to be a suitable replacement for FMN and NADPH can be used as a substitute for NADH. The apparent Km for ferric citrate, NADH and FMN was 1.9 nMm, 0.4 μM and 0.1 MM, respectively. We observed that salts of Ni2+, Co2+, Cu2+, Zn2+, TeO3 2−, CrO4 2−, SeO3 2− and SeO4 2− inhibited the reduction of Fe3+ by the fungal cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
