Abstract
Sun overexposure leads to higher risk of photoaging and skin cancer. The contribution of infrared (IR) and visible light (VIS) radiation is currently being taken into account in their pathogenesis. Erythema, hyperpigmentation, genotoxicity or the increase of matrix metalloproteinases (MMPs) expression are some of the effects induced by these types of radiation. Extracts of various botanicals endowed with antioxidant activity are emerging as new photoprotective compounds. A natural extract from Polypodium leucotomos (Fernblock®, FB) has antioxidant and photoprotective properties and exhibits a strong anti-aging effect. In this study, we evaluated the protective capacity of FB against the detrimental effects of infrared A (IRA) and VIS radiation in human dermal fibroblasts. We analyzed the effects of FB on the morphology, viability, cell cycle and expression of extracellular matrix components of fibroblasts subjected to VIS and IRA. Our results indicate that FB prevents cell damage caused by VIS and IRA. Moreover, it reduces the increase in MMP-1 and cathepsin K expression induced by both VIS and IRA radiation, and curbs alterations in fibrillin 1, fibrillin 2 and elastin expression. All these findings support FB as a feasible approach to prevent or treat skin damage caused by IRA or VIS exposure.
Highlights
Skin aging is a complex biological process characterized by a progressive loss of its physiological integrity
We have evaluated the protective capacity of FB against the detrimental effects induced by IR and visible light (VIS) radiation in human dermal fibroblasts
We report that FB prevents the changes induced by water-filtered IR-A (wIRA) and VIS on the morphology, viability and cell cycle of human fibroblasts, as well as on the expression of different extracellular matrix (ECM) components including matrix metalloproteinases (MMPs)-1, Cathepsin K (CTSK), fibrillins 1 and 2 and elastin
Summary
Skin aging is a complex biological process characterized by a progressive loss of its physiological integrity. We report that FB prevents the changes induced by wIRA and VIS on the morphology, viability and cell cycle of human fibroblasts, as well as on the expression of different ECM components including MMP-1, CTSK, fibrillins 1 and 2 and elastin. After FB treatment though, both FBN1 and FBN2 expression was generally increased after either VIS or wIRA radiation These results are in line with those reported in 2009 by Philips et al [31], where UV light had an inhibitory effect on fibrillins of keratinocytes, and P. leucotomos stimulated the expression of fibrillins in both non-UV-exposed and UV-exposed keratinocytes. We report that FB prevents the changes induced by wIRA and VIS on the morphology and viability of human fibroblasts, as well as on the expression of different ECM components including MMP-1, CTSK, fibrillins 1 and 2 and elastin. Our results suggest that FB could be a preventive treatment against skin cellular damage and ECM alterations caused by VIS and wIRA radiation, and could be beneficial in preventing photoaging
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