Fermentative Quality of Guineagrass Silage by Using Fermented Juice of the Epiphytic Lactic Acid Bacteria (FJLB) as a Silage Additive

S Bureenok,T Namihira,Y Kawamoto,S Mizumachi,T Nakada,M Tamaki

doi:10.5713/ajas.2005.807

Abstract

This experiment examined the characteristics of fermented juice of epiphytic lactic acid bacteria (FJLB) prepared by the addition of glucose, sucrose and molasses as a fermentation substrate. The effect of FJLB on the fermentative quality and changes in chemical composition during fermentation of guineagrass silage were also investigated. The pH value of the silages treated with FJLB rapidly decreased, and reached to the lowest value within 7 days of start of fermentation, as compared to the control. The number of lactic acid bacteria (LAB) in the treated silages increased for the first 3 days, thereafter the number of LAB declined gradually up to the end of the experiment. Silages treated with FJLB had larger populations of LAB than the control. Ammonia-nitrogen production increased throughout the ensiling period, which in the control and no-sugar added FJLB silages were higher than the other treated silages. Lactic acid levels varied with the time of ensiling and among the silage treatments. For any sugar FJLB treated silages, the lactic acid increased initially, and then slightly reduced to less than 50 g/kg of dry matter until 49 days after ensiling, except the silage treated with glucose added FJLB. Nevertheless, lactic acid content of the control decreased constantly from the beginning of ensiling and was not found after 35 days. Moreover, acetic acid content increased throughout the ensiling period. All the FJLB treated silages had significantly (p<0.05) lower pH and ammonia-nitrogen content, while significantly (p<0.05) higher lactic acid content and V-score value compared with the control. This study confirmed that the applying of FJLB with any sugar substrate improved fermentative quality of silage. (Asian-Aust. J. Anim. Sci. 2005. Vol 18, No. 6 : 807-811)

Highlights

Ensiling has been known as a method to preserve the This study is purposed to clarify if the applying fermented juice of epiphytic lactic acid bacteria (LAB) (FJLB) with moist crops by controlling anaerobic fermentation
Been well recognized that the epiphytic LAB plays an important role on silage fermentation, the number of FJLB preparation epiphytic LAB on the standing crop is tiny and variable The FJLB was prepared from guineagrass (Panicum (Muck, 1990; Lin et al, 1992), and found to be lower than maximum Jacq cv
Many researchers have reported that manipulating numbers of the epiphytic LAB can be Silage making improved by using fermented juice of epiphytic LAB (FJLB) Guineagrass was harvested at flowering stage on as a silage additive obtained by macerating crop silage with October 22, 2001 in the experiment field, University of the water and anaerobic incubating for 2 days

Summary

INTRODUCTION

Lactic acid bacteria (LAB) is dominant in the fermentation, and the activity of clostridia is restricted Many researchers have reported that manipulating numbers of the epiphytic LAB can be Silage making improved by using fermented juice of epiphytic LAB (FJLB) Guineagrass was harvested at flowering stage on as a silage additive obtained by macerating crop silage with October 22, 2001 in the experiment field, University of the water and anaerobic incubating for 2 days. The number of lactic acid bacteria (LAB) in FJLB and the extract of crop material and silages were determined by counting the colony forming unit (CFU) after incubation on GYP-CaCO3 agar plate at 35°C for 3 days (Kozaki et al, 1992). Statistical analysis Data obtained on chemical composition of silage ensiled for 49 days were analyzed using the GLM procedure of SAS and the significance of the difference among treatment means was determined by the Duncan’s multiple-range test (SAS, 1998)

RESULTS

DISCUSSION

CONCLUSION