Fermentative production of L-arginine. II. Mechanism of L-arginine production by L-arginine-producing mutants of Corynebacterium glutamicum.

Abstract

Regulatory properties of the arginine biosynthetic enzymes in the l-arginine producers of Corynebacterium glutamicum were examined. d-Serine-sensitive l-arginine producer, DSS–8, had derepressed level of l-arginine biosynthetic enzymes, but had an N-acetylglutamokinase, the key enzyme of l-arginine biosynthetic pathway, sensitive to the feedback inhibition by l-arginine. d-Serine-sensitive and d-arginine-resistant l-arginine producer, KY10479, had an N-acetylglutamokinase released from the feedback inhibition by l-arginine.Implications of these results are discussed in relation to l-arginine productivity, d-serine sensitivity, l-arginine analog resistance and regulation of l-arginine biosynthesis in these mutants.