Abstract
Streptococcus sanguis, Strep. mutans, Strep. salivarius and Strep. bovis were grown anaerobically in continuous culture at pH 7.0 under conditions of glucose-limitation and nitrogen-limitation in complex and in chemically defined media. Fermentation products in the cultures were analysed and the yield of bacterial mass determined. All streptococci formed lactate as the main fermentation product when grown in a medium containing a surplus of glucose with the nitrogen source limiting growth. When grown in a glucose limiting medium, the main fermentation products formed by Strep. sanguis and Strep. mutans were ethanol, acetate and formate. Whereas Strep. salivarius and Strep. bovis formed some ethanol, acetate and formate, but mainly lactate. There was a significantly higher yield of bacterial mass under conditions of glucose-limitation than under conditions of nitrogen-limitation.
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