Fermentation of soybean meal-hydrolysates as the medium that treated by papain enzyme with Saccharomyces cerevisiae for biomass production

Abstract

The purpose of this study was to determine the effect of soybean meal (SBM) media processing using the papain enzyme on the amino acid content and its effect on yeast biomass production. The study was divided into 2 experimental phases, first test: effect of papain enzyme hydrolysis test on amino acid in soybean meal that treated by boiling and non-boiling medium, second test: yeast biomass production was cultivated using soybean meal hydrolysates as medium with 3 treatments, namely control (- soybean meal), with soybean meal (-enzyme) and soybean meal (+enzyme). Data were analyzed using a completely randomized design with ANOVA and post hoc analysis using Tukey’s multiple range test. From the analysis, it was found that the addition of papain enzymes supplemented on soybean meal medium was affected on crude protein and amino acids composition (L-Arginine, L-proline, L-methionine, and L-phenylalanine). Enzyme treatment on SBM as substrate was increased the yeast biomass compared to control (P<0.01). In conclusion, enzyme treatment on soybean meal was improved the fermentation performance of yeast biomass production