Fermentation of sago starch to biobutanol in a batch culture using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

Abstract

Acetone–butanol–ethanol (ABE) was produced in Clostridium saccharoperbutylacetonicum N1-4 using sago starch as a substrate, and the effects of nutrient supplementation on ABE production were studied. Using sago starch as a substrate led to higher butanol production in C. saccharoperbutylacetonicum N1-4 when compared to other starchy materials as substrates. TYA medium supplemented with sago starch led to enhanced ABE and butanol production; however, P2 medium improved ABE production more than TYA medium. The highest ABE and butanol production was achieved with P2 medium supplemented with 70 g/L of sago starch. Using these fermentation conditions, ABE and butanol were produced at 16.65 and 9.83 g/L, respectively, and productivity was 0.15 g/L h for ABE and 0.09 g/L h for butanol. Using P2 medium supplemented with either 50 g/L enzymatically hydrolyzed sago starch or 70 g/L sago starch without hydrolysis, 9.81 and 9.83 g/L butanol were produced, respectively. These results reveal the importance of media formulation in the biobutanol fermentation of sago starch by C. saccharoperbutylacetonicum N1-4.