Abstract

Fermentation of Pretreated Herbaceous Cellulosic Wastes to Ethanol by Anaerobic Cellulolytic and Saccharolytic Thermophilic Clostridia

Highlights

  • Lignocellulosic waste streams from agriculture, paper and wood industry are renewable, plentiful and low-cost raw materials that can be used for large-scale production of liquid and gaseous biofuels

  • 65 new strains of anaerobic thermophilic cellulolytic and saccharolytic Clostridia were isolated from different wetlands and hot springs in Georgia

  • At the stage most, effective strains were used for the development of syntrophic mixed-culture for direct conversion of plant biomass with improved ethanol productivity [1]

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Summary

Introduction

Lignocellulosic waste streams from agriculture, paper and wood industry are renewable, plentiful and low-cost raw materials that can be used for large-scale production of liquid and gaseous biofuels. Fermentation of mechanically pretreated wheat straw and corn stalks was done under oxygen free nitrogen environment in thermophilic conditions (T=550C) and pH 7.1. Xylan, xylose and glucose were determined using standard methods. Cellulolytic and saccharolytic bacteria strains degraded mechanically pretreated herbaceous cellulosic wastes and fermented glucose and xylose to ethanol, acetic acid and gaseous products like hydrogen and CO2.

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