Abstract
The cellulolytic enzymes from Penicillium genus showed well-balanced amounts of cellobiohydrolase, endo-cellulase and β-glycosidase. The Fermentation condition of Penicillium purpurogenum HBZ003 from Mangrove for 1,4-β-D-cellobiohydrolase activity was investigated. The optimum fermentation condition of Penicillium purpurogenum HBZ003 was as follows. The medium was composed of 1.6% bran, 0.4% CMC, 0.5% (NH4)2SO4, 0.4% KNO3, 0.6%NaCl, 0.03% CaCl2 and 0.05% Tween 60, and adjusted to initial pH 4.0, and inoculated with 10% seed and cultivated at 160 r/min and 30 °C for 5d. The 1,4-β-D-cellobiohydrolase activity reached 5.54 U with the ratio of 1,4-β-D-cellobiohydrolase, endo-cellulase and β-glycosidase activity being 1:3.26:5.09.
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