Fenoxycarb exposure affects antiviral immunity and HaNPV infection in the cotton bollworm, Helicoverpa armigera.

Abstract

Application of insect growth regulators (IGR) is a good option for insect pest management because of their fewer adverse effects on humans and domestic animals. These compounds are capable of interfering with normal growth and development by mimicking the actions of hormones such as juvenile hormone (JH) or ecdysone. The effect of JH and its analogs on some aspects of insect immunity has been determined, yet their possible effects on antiviral immunity response has not been investigated yet. Considering the importance of antiviral response in viral replication, in this study the effects of the JH analog (JHA), fenoxycarb on the antiviral immunity pathway core genes [i.e. micro (mi)RNA, small interfering (si)RNA and apoptosis] of Helicoverpa armigera (Hubner) larvae were investigated. The effect of fenoxycarb on the susceptibility of the larvae to H.armigera nuclear polyhedrosis virus (HaNPV) also was assessed. The results showed that the transcription level of miRNA (Dicer1, Ago1), siRNA (Dicer2, Ago2) and apoptosis (Caspase1, Caspase5) core genes in H.armigera larvae were decreased significantly after 24, 48 and 96 h feeding on a diet containing lethal and sublethal doses of fenoxycarb. Moreover, the mortality rate to HaNPV in the larvae treated with fenoxycarb increased compared to the control, leading to an increased replication of HaNPV. Together, our results suggest that the antiviral immune system could be modulated by JHA and facilitate HaNPV replication in the larvae, increasing the mortality rate of the insect larvae. Understanding the effect of JHA on antiviral immunity is an important step toward the process of exploiting JHAs and viral pathogens to control insect pests. © 2022 Society of Chemical Industry.