Abstract

Laser synthesis of fluorescent species from biomolecules in living cells and tissues offers unique capabilities for fluorescent bioimaging, yet little is known about its mechanisms and characteristics of products. We examine synthesis of fluorescent products from water solution of L-lysine upon irradiation by trains of femtosecond laser pulses with varying parameters. We demonstrate that irradiation products contain nanoscale carbon-based fluorescent particles (carbon dots) with multi-colour and excitation-dependent emission. Morphology, chemical composition and fluorescent characteristics of irradiation products strongly depend on laser pulses parameters.

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