Abstract
In this work highly localized femtosecond laser ablation is used to dissect single axons within a living Caenorhabditis elegans (C. elegans). We present a multimodal imaging methodology for the assessment of the collateral damage induced by the laser. This relies on the observation of the tissues surrounding the targeted region using a combination of different high resolution microscopy modalities. We present the use of Second Harmonic Generation (SHG) and Polarization Sensitive SHG (PSHG) to determine damage in the neighbor muscle cells. All the above is done using a single instrument: multimodal microscopy setup that allows simultaneous imaging in the linear and non-linear regimes and femtosecond-laser ablation.
Highlights
Nowadays lasers are one of the most powerful and versatile tools in the biomedical field
We showed how the combination of confocal fluorescence microscopy simultaneously with Laser-scanned Transmitted light microscopy (LT) provides a powerful tool for the real-time observation of the surgery with high resolution and for the study of the different dynamical processes happening during the procedure [4]
We present the use of a combination of linear and nonlinear high-resolution imaging techniques, all of them integrated in a single multimodal optical workstation, to monitor any change induced to the tissues around the operated axon [15]
Summary
Nowadays lasers are one of the most powerful and versatile tools in the biomedical field Apart from their use in macro and microscopic imaging techniques, their application in the noninvasive modification of biological samples is of particular importance. For these purposes, ultrashort pulsed lasers employing near infrared wavelengths have been shown to be the ideal tool when a very controlled and precise modification is required. Ultrashort pulsed lasers employing near infrared wavelengths have been shown to be the ideal tool when a very controlled and precise modification is required These lasers have the ability to induce nonlinear photoionization and thereby to confine interaction to the focal volume (,1 femtoliter) of a tightly focused beam [1]. This is opening up a whole new window for laser nanosurgery as a non-invasive powerful surgery technique that can be applied in a vast range of biomedical areas
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
