Femto-second laser (FSL) techniques and consistency in corneal surgery: experimental study

Abstract

ObjectiveTo investigate the feasibility, effectiveness, and reproducibility of femtosecond laser (FSL) technology in different corneal cuts. DesignExperimental study at the Brandon Regional Health Centre. ParticipantsTwenty two Human Cadaver eyes donated for research and supplied by the eye bank of Manitoba university. MethodsIn this experimental study, the effectiveness and reproducibility of FSL were investigated in different corneal cuts. The corneas were divided into 5 groups: group I for penetrating keratoplasty; group II for anterior lamellar keratoplasty; group III for corneal pockets for inlays; group IV for intracorneal ring tunnels; and group V for corneal cross-linking pockets. Our primary endpoint was the repeatability of planned versus obtained thickness and diameter of the cuts with FSL. A secondary endpoint was the effectiveness and ease of dissection, rated from 0–4, with 4 being the easiest and 0 the most difficult to dissect. ResultsThere was a high correlation between planned and obtained thickness (r = 0.997, p < 0.001) and diameter (r = 0.998, p < 0.001). Both were statistically significant. Linear regression analyses showed that the obtained thickness of corneal cuts in micrometers was significantly predicted by the planned thickness of corneal cuts in micrometers (β = 0.996, t = 56.47, p < 0.001). Likewise, the obtained diameter of corneal cuts in millimeters was significantly predicted by the planned diameter of corneal cuts in millimeters (β = 0.971, t = 70.85, p < 0.001). The surgeons gave 15 out of a total of 22 corneas (68.2%) the maximum score for ease of dissection (Grade 4), where no adhesions were found and the dissection was smooth and steady. Five corneas out of a total of 22 (22.7%) were given a score of 3 of 4 where minor adhesions were found. Two corneas out of a total of 22 (9.1%) were given a score of 2 of 4 where >1 adhesion was found, with some difficulty in separating the cut cornea from the bed. No corneas were given grade 1 or grade 0. No significant differences were obtained for the ease of dissection among the 5 surgery types using Kruskal–Wallis H test (H [4] = 4.971, p > 0.05). ConclusionThe measured corneal-cut geometry correlated well with laser settings in a variety of full- and partial-thickness FSL corneal patterns, including different depths and diameters. This reproducible efficacy and measurement accuracy of the planned versus obtained cuts could have a favourable result on a variety of corneal surgeries. It also yields a favourable ease of dissection of the cut lenticule from the stromal bed.