Females face more positive plant-soil feedback and intersexual competition under adequate nitrogen conditions compared to males in Populus cathayana

Abstract

Plant-soil feedback (PSF) and competition influence plant performance, community structure and functions. However, how nutrient availability affects the interaction of PSF, sexual competition and coexistence in dioecious plants is poorly understood. In this study, the strengths of PSF and sexual competition, and their responses to nutrient availability were assessed in dioecious Populus cathayana using a garden experiment. We found that PSF reduced but did not eliminate the inequal sexual competition at low nitrogen (N) availability. Intersexual competition and nutrient limitation induced more negative PSF, which promoted sexual coexistence. PSF and competition were rather related to sexual dimorphism. Female plants experience more positive PSF and intersexual competition under adequate N conditions compared to males; the contrary was true with low N supply. Furthermore, the stability of root exudate networks and soil nutrient availability reflects the possibility of sexual coexistence regulated by PSF. Intersexual interaction promote more stable root exudate profiles and more saccharide secretion at low N supply. Meanwhile, the increased soil N and P mineralization in females with cultivated males explained the possible coexistence between females and males at low nutrient availability. Thus, these results indicate that soil biota can mitigate differences in sexual competitiveness and improve the stability of root exudate networks, consequently promoting sexual coexistence at low nutrient availability.