Female and male green monkey liver estrogen receptor

Abstract

Adult female and male green monkey liver cytosol contains tritiated 17β-estradiol ([ 3H]E 2) binding sites. The binding of [ 3H]E 2 to these sites is reduced substantially by diethylstilbestrol. Unlike adult male rate liver cytosol, neither male nor female green monkey liver cytosol contains moderate affinity high capacity [ 3H]E 2 binding sites. The green monkey liver cytosol [ 3H]E 2 sites are precipitated by ammonium sulfate at 30% of saturation. The properties of the redissolved ammonium sulfate precipitated female and male [ 3H]E 2 binding sites are similar: they have a high [ 3H]E 2 affinity, a low [ 3H]E 2 capacity, are estrogen specific, and are sensitive to sulfhydryl inactivation and protease digestion. Although the female and male sites are similar, there appears to be a 2- to 3-fold sex difference in the affinity (female K d , 0.8 to 1.3 × 10 −10 M; male K d , 0.4 × 10 −10M) and capacity (female, 2.3 to 3.7 fmoles/mg of liver; male, 1.2 fmoles/mg of liver)of the [ 3H]E 2 binding sites. Accordingly, adult female and male green monkey liver cytosol contains putative estradiol receptors.