Abstract
Cancer stem cells were identified in a feline mammary carcinoma cell line by demonstrating expression of CD133 and utilising the tumour sphere assay. A population of cells was identified that had an invasive, mesenchymal phenotype, expressed markers of pluripotency and enhanced tumour formation in the NOD-SCID mouse and chick embryo models. This population of feline mammary carcinoma stem cells was resistant to chemotherapy and radiation, possibly due to aberrant activation of the ATM/p53 DNA damage pathway. Epithelial–mesenchymal transition was a feature of the invasive phenotype. These data demonstrate that cancer stem cells are a feature of mammary cancer in cats.
Highlights
Mammary tumours frequently develop in female domestic cats and are the third most commonly reported cancer in this species (Hayes and Mooney, 1985)
We used a feline mammary carcinoma (FMC) cell line to characterise feline mammary cancer stem cell (CSC) in terms of increased expression of specific stem cell markers, higher potential for mammosphere formation, invasiveness and resistance to radiation and chemotherapy compared to the bulk tumour population
A FMC cell line was used to characterise feline mammary CSCs through expression of specific stem cell markers, higher potential for mammosphere formation, invasiveness and resistance to radiation and chemotherapy compared to the bulk tumour population
Summary
Mammary tumours frequently develop in female domestic cats and are the third most commonly reported cancer in this species (Hayes and Mooney, 1985). Cats that are ovariohysterectomised
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