Abstract
A feeding chamber and diet were developed for rearing Bemisia argcntifolii (Bellows & Perring) from eggs to 4th in star under aseptic conditions. The feeding apparatus consisted of a plastic diet chamber covered with stretched Parafilm that was held in place by another piece of plastic and binder clips. Surface-sterilized eggs were placed on the Parafilm, and the chamber was incubated in a petri dish at 90-95% RH, 25-27ºC and a photoperiod of 16:8 (L:D) h. The most successful diet consisted of 5% yeast extract in 30% sucrose. Developmental times of 1st-, 2nd-, and 3rd-instar nymphs were 4.6, 3.4, and 4.2 d, respectively, which is comparable to development times on plants. Although whitefly nymphs can be reared on this diet to 4th in star, complete development to the adult stage was not achieved. The utility of the feeding system was demonstrated in bioassay with Ivermectin.
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