Feeding activity, absorption efficiency and suspension feeding processes in the ascidian, Halocynthia pyriformis (Stolidobranchia: Ascidiacea): responses to variations in diet quantity and quality

Abstract

The benthic suspension feeding ascidian, Halocynthia pyriformis (Rathke, 1806), is often exposed to high concentrations of resuspended sediment in the Bay of Fundy. Resuspended sediment can change diet quantity and quality that may alter the ascidian's ability to feed and gain energy. The feeding activity of H. pyriformis exposed to bottom sediment was examined using standard physiological techniques and video endoscopy. Ascidians were exposed to natural seston plus additions of bottom sediment ranging in concentration from 0 to 46 mg l(-1). For each sediment concentration, clearance rate, ingestion rate, and retention efficiency of the ascidians was estimated using flow-through feeding chambers. Samples of suspended particles and feces were collected to estimate absorption efficiency and absorption rate. Results indicate that with increasing sediment concentration, ingestion rate increased to a constant level, absorption rate increased linearly despite a logarithmic decrease in absorption efficiency, and the retention of small particles (2-5 &mgr;m) increased while retention of larger particles (5-15 &mgr;m) decreased. As sediment concentration increased, squirting frequency increased and diameter of the siphon was reduced. Endoscopic observation of feeding structures and processes and the measurement of particle velocity was performed on ascidians exposed to 0 and 10 mg l(-1) of bottom sediment. An increase in squirting frequency at the high concentration facilitated the rejection of unwanted material and altered the structure and transport velocity of mucus. Mucus velocity was five times slower at 10 mg l(-1) than at 0 mg l(-1), however, the overall distance of mucus travel and the probability of clogging was reduced at 10 mg l(-1). H. pyriformis appears to compensate for episodic changes in the quantity and quality of available food particles by altering siphon-opening diameter, squirting frequency, structure and transport of mucus, and retention efficiency to maintain constant clearance rates.