Abstract
THE search for rate-controlling enzyme systems in the overall synthesis of DNA has led a number of investigators to study the reactions in the sequence from Tdr to dTTP. Little duplication of effort has occurred despite the similarity in overall objectives. The work reported by Dr Smellie difiers from our work in terms of source of enzyme, reaction components, separation of products, method of quantitating the data, and problems emphasized [a]. Dr Smellie utilized extracts from the Landschutz ascites tumor cells while our work was done with extracts from the NlSl strain of cells grown in tissue culture in vigorously shaken suspensions in a modified Eagle medium. This strain was derived from Novikoff hepatoma cells and in addition to being the richest source of Tdr kinase and TMP kinase found, it is also notably lacking in side reactions that utilize Tdr. In the following scheme
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