Abstract
The object of the present work was to optimise inoculum size and nitrogen source for rapid production of xanthan by Xanthomonas campestris by fed-batch fermentation. The fermentation period for production of xanthan by X. campestris decreased from 72 h to 48 h by using 30% (v/v) inoculum instead of the usual 10% level. Among various nitrogenous substances tested in the medium having the C/N ratio of 28, the best concentration of 1.9% (w/v) of xanthan with a viscosity of 10,000 cP was obtained for KNO3 medium. Intermittent harvests operated for three cycles of 48 h each, using 30% inoculum resulted in xanthan broth of high viscosity (9000 cP) with a concentration of 1.9% and a yield of 68% (w/w). Potassium nitrate was a better suited nitrogen source for the medium used for rapid production of xanthan.
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